Equipment

This is an abbreviated list of equipment that Faculty, Staff, and students at Puget Sound can utlize. The equipment listed on this page is also available to outside users. If you are not a member of the Puget Sound community and you are interested in using one of these instruments please see our Outside Users page.

If you have questions about other equipment listed on our main page, please contact the Science Core Facility Technician.

 

Hitachi S3400N Variable Pressure Scanning Electron Microscope

Hitachi S3400N Variable Pressure Scanning Electron Microscope

The S3400N is a powerful, yet user-friendly SEM  through newly developed electron optical and automated functions. The famous Hitachi image quality convinces at high and low beam energies, and optimized detector technology provides maximum information from your sample. Variable chamber pressure allows chargeup-free observation of any sample without special preparation techniques such as coating.

The S3400N at Puget Sound is also equipped with Energy Dispersive X-ray Spectrometry, and a Cathode Luminescence Spectrometer.

 

 Ziess CEM 902 Transmission Electron Microscope

 

The Zeiss CEM 902 is a thermionic (tungsten) transmission electron microscope (TEM) with a line resolution of 0.34 nm and a point resolution of 0.5 nm. It operates in normal, diffraction, and low dose modes at 50 or 80 kV. Its magnification range is 150x to 400,000x. Images may be acquired with a Hamamatsu high resolution digital camera.

 

 

 Nikon D-Eclipse C1 Confocal Microscope

The Eclipse C1 is a modular confocal microscope system from Nikon.

The C1 at Puget sound is mounted on a Nikon Ti inverted microscope on a vibration isolation table. It comes equipped with 3 laser lines: 405 nm, 488 nm, and 543nm.

We have 4 available objectives; Plan Apo 10X DL Dry, Plan Apo VC 20X Dry, Plan Apo VC 60X WI Water Correction Ring, and Plan Apo VC 100X oil

 

 

BioRad CFX96 Real-Time PCR Detection System

 

The BioRad CFX96 is a modular thermal cycler platform mounted on a C1000 thermal cycler base that can accomodate a 96-well plate. The CFX96 system has high ramp rates to target temperatures, which significantly reduces run times. In addition, the sytem can discriminate up to five targets in a single reaction well for multiplexing experiments. Data is analyzed by the powerful CFX Manager software.

 

 

GenePix 4100A Microarray Scanner

The GenePix Personal 4100A scanner has the same high-performance quantitative imaging as the benchmark GenePix 4000B scanner. Scans microarray slides sequentially at up to two wavelengths. Has precisely controlled laser temperature and pixel-by-pixel laser correction to compensate for normal variation in laser intensity. Uses 16-bit digitization. Has a dynamic detection range of four orders of magnitude. Has two standard filters. Includes line-averaging mode for enhanced signal-to-noise ratio. Offers two scanning modes: Preview Scan at 40-μm resolution and Data Scan at 5, 10, 20, 40, 60, 80, and 100-μm resolutions. Full integration with GenePix Pro software.

 

LI-COR Odyssey Fc Dual Mode Imaging System

The Odyssey Fc advances Western blot technology by combining the rich history of chemiluminescence with the power and sensitivity of the LI-COR FieldBrite™XT optical system for exceptional dual-mode detection for both chemiluminescent and infrared fluorescent imaging. Applications include: Western Blots (chemiluminescent, Two-Color Infrared, and In-Gel), Protein Detection with Coomassie-Stained Gels, and Nucleic Acid Detection (SYBR Family, and Syto 60 DNA stains).